60Co irradiation of Shiga toxin (Stx)-producingEscherichia coliinduces Stx phage
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چکیده
منابع مشابه
Shiga Toxin (Stx) Type 1a Reduces the Oral Toxicity of Stx Type 2a
BACKGROUND Shiga toxin (Stx) is the primary virulence factor of Stx-producing Escherichia coli (STEC). STEC can produce Stx1a and/or Stx2a, which are antigenically distinct. However, Stx2a-producing STEC are associated with more severe disease than strains producing both Stx1a and Stx2a. METHODS AND RESULTS To address the hypothesis that the reason for the association of Stx2a with more sever...
متن کاملComparative Genomics and stx Phage Characterization of LEE-Negative Shiga Toxin-Producing Escherichia coli
Infection by Escherichia coli and Shigella species are among the leading causes of death due to diarrheal disease in the world. Shiga toxin-producing E. coli (STEC) that do not encode the locus of enterocyte effacement (LEE-negative STEC) often possess Shiga toxin gene variants and have been isolated from humans and a variety of animal sources. In this study, we compare the genomes of nine LEE-...
متن کاملPresence of activatable Shiga toxin genotype (stx(2d)) in Shiga toxigenic Escherichia coli from livestock sources.
Stx2d is a recently described Shiga toxin whose cytotoxicity is activated 10- to 1000-fold by the elastase present in mouse or human intestinal mucus. We examined Shiga toxigenic Escherichia coli (STEC) strains isolated from food and livestock sources for the presence of activatable stx(2d). The stx(2) operons of STEC were first analyzed by PCR-restriction fragment length polymorphism (RFLP) an...
متن کاملShort-tailed stx phages exploit the conserved YaeT protein to disseminate Shiga toxin genes among enterobacteria.
Infection of Escherichia coli by Shiga toxin-encoding bacteriophages (Stx phages) was the pivotal event in the evolution of the deadly Shiga toxin-encoding E. coli (STEC), of which serotype O157:H7 is the most notorious. The number of different bacterial species and strains reported to produce Shiga toxin is now more than 500, since the first reported STEC infection outbreak in 1982. Clearly, S...
متن کاملRelationship between stx genotype and Stx2 expression level in Shiga toxin-producing Escherichia coli O157 strains.
To determine the expression level of Shiga toxin (Stx) 2-related toxins (Stx2 and Stx2c) produced by each of 33 Stx-producing Escherichia coli (STEC) O157 strains, stx2 and stx2c mRNAs (stx2-related mRNA) were measured using real-time PCR with primers that recognize sequences common to stx2 and stx2c. The amount of Stx2 and Stx2c protein was measured using a reversed passive latex agglutina...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2003
ISSN: 0378-1097,1574-6968
DOI: 10.1016/s0378-1097(03)00259-3